To screen pH as a crystallization variable using Tacsimate one can mix together different ratios of different pH Tacsimate solutions. Refer to the pdf "Mixing Tacsimate Stocks to Screen pH" on this page. Or, one can add a buffer to the crystallizaiton reagent containing Tacsimate. Recommended final buffer concentration in the reagent well is 0.05 to 0.1 M.
TacsimateA pH titrated mixture of organic acids. 1.8305 M Malonic acid, 0.25 M Ammonium citrate tribasic, 0.12 M Succinic acid, 0.3 M DL-Malic acid, 0.4 M Sodium acetate trihydrate, 0.5 M Sodium formate, 0.16 M Ammonium tartrate dibasic. pH adjusted using NaOH
Measured pH of 100% Tacsimate is 4.0 at 25°CMeasured pH of 100% Tacsimate is 5.0 at 25°CMeasured pH of 100% Tacsimate is 6.0 at 25°CMeasured pH of 100% Tacsimate is 7.0 at 25°CMeasured pH of 100% Tacsimate is 8.0 at 25°CMeasured pH of 100% Tacsimate is 9.0 at 25°C
