HEPES
Synonyms: 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid or 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid or N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)C8H18N2O4SMr 238.31CAS Number [7365-45-9]EC Number 230-907-9Beilstein Registry Number 883043Merck 14,4654RTECS TL6809000MDL Number MFCD00006158PubChem Substance ID 24895616Purity ≥ 99.5%pKa 7.5 at 25°C
Useful pH range of HEPES buffer 6.8 -8.2
Titrate HR2-585 to the desired pH between 6.8 - 8.2 using NaOHHR2-585 Measured pH Range: 5.4 - 5.5 at 25°CHR2-585 Measured Conductivity Range: 160.1 - 236.9 μS/cm at 25°CHR2-585 Measured Refractive Index Range: 1.37093 - 1.37131 at 20°C
HR2-785 is titrated to pH 7.0 using NaOHHR2-785 Measured Conductivity Range: 5.8 - 7.0 mS/cm at 25°CHR2-785 Measured Refractive Index Range: 1.37133 - 1.37176 at 20°C
HR2-729 is titrated to pH 7.5 using NaOHHR2-729 Measured Conductivity Range: 11.6 - 13.6 mS/cm at 25°CHR2-729 Measured Refractive Index Range: 1.37177 - 1.37290 at 20°C
Insoluble Matter 0.01% maxDNase None DetectedRNase None DetectedProtease None DetectedHeavy Metals 1 ppm maxAs 5 ppm maxCu 5 ppm maxFe 5 ppm maxPb 5 ppm maxSO4 0.005% max
